Isolation and Screening of Zinc Solubilizing Rhizosphere Bacteria and Their Impact on Growth of Vigna unguiculata (Black Eyed Pea Plant)
Keywords:
Zinc as essential trace element, Zinc solubilizing rhizosphere bacteria, Solanum lycopersicum (Tomato), Zea maize (Maize), Sorghum bicolor (Sorgum), Experimental Vigna unguiculata (L.) Walp. (Black eyed pea) Plant, Plant Growth Promoting Rhizosphere (PGPR) Bacteria, BiofertilizerAbstract
Zinc is one of the eight essential trace elements that plants need for normal growth and
reproduction. Plants require Zinc in small but critical concentrations. Zinc is present in soil in
their complex organic form, but plants require their simple ionic form for fulfilling their
requirements. Soil microorganisms play a crucial role in solubilization and mobilization of
this element for plant growth and increase crop yield. In the present investigation, there were
5 Zinc Solubilizing Bacterial isolates (ZnSB) obtained from Solanum lycopersicum (Tomato),
Zea maize (Maize), and Sorghum bicolour (Sorgum) rhizosphere soil samples, using growth
medium supplemented with insoluble source of Zinc such as Zinc oxide (ZnO) 0.1%. All
ZnSB isolates were further tested for their identification up to genus level in VITEK 2
automated microbiology system and results revealed that they all were Bacillus thuringiensis.
They are further investigated for their biochemical activities and effect on Vigna unguiculata
(Black eyed pea) plant’s growth. Experiment revealed that all 5 ZnSB isolates were
promising in exhibiting almost all Plant growth promoting characters. As Plant Growth
Promoting Rhizosphere (PGPR) bactetia are environmental friendly and offer sustainable
approach to increase production of crops and health. Therefore, these isolates can be utilized
for biofertilizer formulation under local agroclimatic conditions which decreases the
application of chemical fertilizers.
Downloads
References
Alexander M. (1997). Introduction to Soil Microbiology. New York, NY: John Wiley and
Sons. [Google Scholar]
Alloway, B. J. (2004). Zinc in Soil and Crop Nutrition. Belgium: International Zinc
Association.
Alloway, B.J., 2008, Zinc in soils and crop nutrition.Second edition, published by IZA and
IFA, Brussels, Belgium and Paris, France.
Anonymous,1993. International Rice Research Institute. IRRI, Phillippines.
Barthalomew, J.W. and Mittewer, J., 1950, A simplified bacterial strain. Stain Tech., 25: 153.
Cappuccino, J. G. and Sherman, N., 1992, Microbiology: A laboratory manual. The
Benjamin/Comings Publishing Company, Inc., California.
Chang, H. B., Lin, C. W., and Huang, H. J. (2005). Zinc induced cell death in rice (Oryza
sativa L.) roots. Plant Growth Regul. 46, 261–266.
Di Simine, C. D., Sayer, J. A. and Gadd, G. M., 1998, Solubilization of Zinc phosphate by a
strain of Pseudomonas fluorescens isolated from forest soil. Biol. Fertile.soils., 28:
-94.
Epstein, E., and Bloom, A. J. (2005). Mineral Nutrition of Plants: Principles and
Perspectives. California: Sinauer Associates.
Fasim, F., Ahmed, N., Parson, R. and Gadd, G.M. (2002).Solubilization of Zinc salts by a
bacterium isolated from air environment of a tannery. FEMS Microbiol.Lett. 213(1-6).
Hughes, M. N. and Poole, R.K., 1989, Metals and microorganisms. Chapman and Hall,
London. pp:412.S
Hoagland, D. R., and Arnon, D. I. (1950).The Water-culture Method for Growing Plants
without Soil. Berkley, CA: California Agricultural Experimental Station Publications;
College of Agriculture; University of California.
Jorgensen, J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and
Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol.1.
Kabata-Pendias, A., and Pendias, H. (2001).Trace Elements in Soils and Plants. London:
CRC Press.
Reyes, R. Y. and Brinkman, R., 1989, The effect of Zinc application and other land and crop
management measure on wetland rice productivity in a Zinc-deficient soil. Philippine.
J. Crop Sci. 61: 7-13.
Singh, B., Natesan, S. K. A., Singh, B. K. and Usha, K., 2005. Improving zinc efficiency of
cereals under zinc deficiency. Current Science. 88(1):36– 44.
“Show foods” Ndb.nal.usda.gov. Retrieved 2014-06-04.
Youssef, M.M.A and Eissa, M.F.M. 2014. Biofertilizers and their role in management of
plant parasitic nematodes. E J Biotechnol Pharm Res. 5: 1-6.
