SCREENING OF ENDOPHYTIC ACTINOMYCETES FOR CELLULOSE DEGRADATION
Keywords:
endophyte, actinomycetes, endoglucanase, cellobiase, FPaseAbstract
Actinomycetes or Actinobacteria are Gram positive, filamentous, spore forming ahigh GC containing(60-75% in their DNA) prokaryotes. Endophytic Actinomycetes are the microbes living in the inner part of plant tissues and important for production of the bioactive molecules. Much of the work on these group were restricted on antibiotic potentiality. In the present study an exploration was carried out with the available six endophytic isolates (from Dept of Biotechnology, Atmiya University Rajkot)for cellulase synthesis by primary Screening and secondary screening. Three types cellulase activity (Endoglucanase- 0.368IUat72h, FPase-0.06IU at 96hand cellobiase-0.321 IU at 96 h ) of incubation periodwere found to be recorded by employing the strain Nocardiopsis alba EA23 Important morphological, biochemical & physiological identification at preliminary level found to reveal both branched and un branched type of actinomycetes whose genus and species identification yet under progress.
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Alharbi, S..A., Arunachalam, C., Murugan, A.M. and Wainwright, M. 2012. Antibacterial activity of actinomycetes isolated from terrestrial soil of Saudi Arabia. Journal of Food, Agriculture & Environment. 10 (2):1093-1097.
Aly A., Debbab A., Proksch P.2011.Appl Microbiol Biotechnoi.90:1829-1845.DOI 10.1007/s00253-011-3270-yBibb, M. J. 2005. Regulation of secondary metabolism in streptomycetes. Current Opinion in Microbiology. 8(2): 208–215.
Bouzigarne, B., Ait Ben Aouamar A.2014. Diversity of plant associated actinobacteria. In: Maheshwari DK, ed. Bacterial Diversity in Sustainable Agriculture, Sustainable Development and Biodiversity1.Switzerland: Springer International Publishing.41-99.
Duddu,M.K. and Guntuku,G. 2015. Isolation, Screening And Characterization of Antibiotic Producing Actinomycetes from Kapuluppada Plastic Waste Dumping Yard, Visakhapatnam. Int J Pharm Pharm Sci. 8(11): 221- 229.
Ghose, T.K . 1987. Measurements of cellulase activities. Pure Appl Chem. 59: 257- 268.
Goodfellow, M. and O’Donnell, A.G., 1989. Search and discovery of industrially significant actinomycetes. In: Microbial Products: New Approaches Baumberg, S., Hunter, I.S. and Rhodes, P.M. (eds.),pp. 343-383. Cambridge University Press, Cambridge.
Gram, C., 1884. The differential staining of Schizomycetes in tissue sections and in dried preparations. Forischritte der Mediccine. 2:185-189.
Kim H.-J., Lee Y.-J., Gao W., Chung C.-H., Son C.-W., Lee J.-W. 2011. Statistical optimization of fermentation conditions and comparison of their influences on production of cellulases by a psychrophilic marine bacterium. Psychrobacteraquimaris LBH-10 using orthogonal array method. Biotechnol. Bioprocess. Eng. 16 542–548.
Ku´zniar, A., Włodarczy, K.K. and Woli´nska, A. 2019. Agricultural and Other Biotechnological Applications Resulting from Trophic Plant-Endophyte Interactions- A Review. Agronomy.9(779):1-22.; Doi:10.3390/agronomy9120779.
Kumar, R. R. and Jadeja,V.J. 2016. Diversity and antibacterial potential of endophytic actinomycetes isolated from medicinal plants of Rajkot, India. Life sciences Leaflets. 79(2016):14-26. Online ISSN No. 0976-1098.
Liang, Yan-Ling, Zhang, Zheng, Wu, Min, Wu, Yuan, Feng, Jia-Xun. 2014. Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1. BioMed Research International.2014.1-13.
Mohan, K. L. and Rajamanickam, U. 2018. Biodiversity of actinomycetes and secondary metabolites –A Review .In an original International Journal of Sciences.5(1):21-27.
Mohanta, Y.K. 2014. Isolation of cellulose degrading actinomycetes and evaluation of their cellulolytic potential. Bioeng Bioscience. 2:1-5.
Passari, A.K., Mishra,V.K., Singh, G., Singh, P., Kumar ,B., Gupta, V.J., Sarma, R.K., Saikia, R., Donovan, A.O. and Singh,B.P.2017.Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production. Scientific Reports.7:11809. Doi:10.1038/s41598-017-12235-4.
